De novo assembly of the perennial ryegrass transcriptome using an RNA-Seq strategy.
BackgroundPerennial ryegrass is a highly heterozygous outbreeding grass species used for turf and forage production.Heterozygosity can affect de-Bruijn graph assembly making de novo transcriptome assembly of species such as perennial ryegrass challenging.Creating a reference transcriptome from a homozygous perennial ryegrass genotype can circumvent